Phosphorescence Lifetime imaging* (PLIM)

Phosphorescence Lifetime Imaging works on a similar principle as fluorescence lifetime imaging (FLIM), but rather makes use of the phosphorescence induced by the excitation rather than the fluorescence. In PLIM the sample is excited for a certain time and the phosphorescence photons coming from the sample are collected, measuring how long the sample remains in an excited state. The common time range in which phosphorescence photons are collected is up to milliseconds.