Macro Serial Blockface Fluorescence Imaging (S-BFI)

Serial block face imaging (SFBI) is a method used to generate 3-dimensional (3D) reconstruction of a sample via serial image acquisition, here on a microtome. The system can detect the endogenous autofluorescence signal of paraffin-embedded samples. Optimization of quality section recovery offers the possibility to develop correlative approaches and multimodal analysis between the 3D dataset with the 2-dimensional (2D) sections that can be stained afterwards.