NORWAY
NorMIC Oslo - Advanced Light Microscopy Node Oslo
The Norwegian advanced light microscopy Node is based on two imaging facilities, one at the University Hospital, Montebello, Oslo and one at the Department of Biosciences, Oslo University at Blindern, Oslo. Main research areas are kinetics of intracellular vesicle traffic, fusion, fission, cell division, cell migration and immune cell interactions in addition to imaging of fixed samples.
Specialties and expertise of the Node
The platform has a focus on live imaging of tissue culture and most light microscopy units are equipped with incubators for stable temperature and CO2. The unit offers expertise and technical assistance in a range of advanced light microscopes including confocal laser scanning instruments, spinning disc confocal, TIRF, as well as access to immuno-EM techniques.
Offered Technologies:
ISIDORe is a Horizon Europe funded project that brings together 154 partners from 32 countries around the world, and is designed to effectively support research on infectious diseases and increase preparedness for pandemic.
| Technologies | Euro-BioImaging | ISIDORe |
| Deconvolution widefield microscopy (DWM) | ✓ | ✓ |
| Laser scanning confocal microscopy (LSCM/CLSM) | ✓ | ✓ |
| Spinning disk confocal microscopy (SDCM) | ✓ | ✓ |
| Structured illumination microscopy (SIM)* | ✓ | ✓ |
| Total internal reflection fluorescence microscopy (TIRF) | ✓ | ✓ |
| Image Scanning microscopy (ISM) | ✓ | ✓ |
| Single Molecula localisation microscopy (SMLM) | ✓ | ✓ |
| Fluorescence Resonance Energy Transfer (FRET) | ✓ | ✓ |
| Fluorescence Recovery After Photobleaching (FRAP) | ✓ | ✓ |
| High-speed Imaging * | ✓ | ✓ |
| TEM of chemical fixed samples (TEM) | ✓ | ✓ |
| Immuno-gold EM on thawed cryo-sections (Tokuyasu-EM) | ✓ | ✓ |
| Immuno-gold EM on resin sections (resin-EM) | ✓ | ✓ |
| Pre-embedding immunolabelling (pre-embed IL) | ✓ | ✓ |
| Pre-embeded CLEM | ✓ | ✓ |
Additional services offered by the Node
- Methodological setup
- Training and technical assistance
- Cell culture facilities
- Wet lab space
- Probe preparation
- Data processing and analysis
- Probe preparation
- Housing facilities
Contact details
Oddmund Bakke
Head of NorMIC-Oslo facility
oddmund.bakke@ibv.uio.no
+4795851479
Andreas Brech
Head of CLEM/EM facility
andreas.brech@rr-research.no