Euro-Bioimaging

SPAIN

Advanced Light Microscopy Node Bilbao Spain

The Bilbao Advanced Light Microscopy Node (ALM@BIO) is a joint initiative of the imaging platforms of the Instituto Biofisika (CSIC, UPV/EHU) and Achucarro Basque Center for Neuroscience, both located in the Science Park of the University of the Basque Country (UPV/EHU) in the metropolitan area of Bilbao. The Node is focused on quantitative imaging of cellular events such as molecular interactions and biophysical studies such as lipid-mediated protein interactions or lipid-order imaging of model membranes and cells; and on the neurobiology of neuron-glia interactions.

Specialties and expertise of the Node

ALM@BIO provides assistance during the whole experimentation process: experiment design, training, image analysis and storage. The Node offers experimental configurations that are not available commercially and home-made application-tailored software with a particular emphasis on membrane-related measurements and neurobioimaging, such as:

Spatio-temporal study of intracellular molecular interactions (FRET-FLIM)
Ligand-receptor binding (FCS)
Lipid-mediated protein interactions (scanning FCS)
Intact-cell imaging of lipid concentration at cellular membranes
Super resolution imaging of primary cells and slices of the CNS
Live imaging of genetically encoded calcium indicatora

Offered Technologies:

Fluorescence correlation spectroscopy (FCS)
Fluorescence cross-correlation spectroscopy (FCCS)
Fluorescence-lifetime imaging microscopy (FLIM)
Fluorescence resonance energy transfer (FRET)
Stimulated emission depletion microscopy (STED)

Instrument highlights

Special features of the instruments at the Bilbao Node are:

Tailor-made set-up for scanning FCCS for the study of lipid-mediated protein interactions
Live cell imaging capabilities: CO2 chamber and resonant scanner
In-house developed analysis software tailored to each application
Stimulated emission depletion microscopy (Gated CW-STED)

Contact details