An open source structured illumination microscopy extension for general fluorescence microscope body
Published: 2023-07-25
Imaging technologies are becoming increasingly complex and ever more expensive, reducing the general accessibility and potential reach of cutting-edge techniques. The Special Edition Virtual Pub “Open Hardware in Imaging,” in collaboration with the Euro-BioImaging Industry Board, will highlight developments from scientists and companies who are committed to making biological & biomedical imaging hardware and software solutions openly available to a wide audience.
When: September 22, 2023, from 13:00-15:00 CEST
Where: Online
At this event, Haoran Wang, Leibniz-Institute of Photonic Technology, Jena, Germany, will present An open source structured illumination microscopy extension for general fluorescence microscope body -(full abstract below). Hear this talk and others like it on September 22!
ABSTRACT
An open source structured illumination microscopy extension for general fluorescence microscope body
Haoran Wang
Leibniz-Institute of Photonic Technology, Jena, Germany
This work demonstrates a structured illumination extension for a general epi-fluorescence microscope. Its low photon-dose and speed makes structured illumination microscopy (SIM) an ideal super-resolution (up to 2x) fluorescence microscopy for live cell imaging. However, the high price tag of commercial and home build devices makes it a rare and exclusive tool not available to a large group of researchers.
In our setup, a digital mirror device (DMD) is used as a spatial light modulator operating at video frame rates. The setup is fully controlled by the open source software "ImSwitch", and thanks to the SIM reconstruction algorithm, the setup can reconstruct the raw images in real time. The self-contained module adapts to many commercial microscope bodies and can be replicated using off-the-shelf tools and hardware to provide high-resolution microscopy techniques on a small budget.
